Theileria annulata, as a pathogenic agent of tropical theileriosis, gives rise annually to serious economic losses in cattle industry of Iran. The carrier cattle, harbouring the latent forms of Theileria annulata, play a major role in infecting tick vectors and in disseminating the infection. The aim of this study was determining the carrier cattle, infected with Theileria annulata in Golestan ...

We report the detection of Theileria annulata, the causative agent of tropical theileriosis, by PCR in blood samples obtained from carrier cattle. The assay employs primers specific for the gene encoding the 30-kDa major merozoite surface antigen of T. annulata. A 721-bp fragment was amplified from blood samples taken monthly from calves experimentally infected with one of four different stocks...

The present study assesses the efficacy of SVANOVIR Theileria annulata-Ab, the first commercial ELISA kit for the diagnosis of Theileria annulata infection in cattle based on a recombinant protein known as T. annulata surface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) assay depending on T. annulata merozoite surface antigen (Tams-1) was applied. A total of 468 blood ...

bovine tropical theileriosis caused by theileria annulata is an economically important disease of cattle. an enzyme linked immunosorbent assay (elisa) was used to determine antibody levels in vaccinated and unvaccinated cattle, us¬ing cellular schizont as antigen and its results were compared with immunofluo-rescent assay (ifa). for this test 126 sera collected (105 vaccinated, 31 not vacci¬nat...

BURRIDGE, M. J., BROWN, C. G. D., AND KIMBER, C. D. 1974. Theileria anndata: Cross-reactions between a Cell Culture Schizont Antigen and Antigens of .East African Species in the Indirect Fluorescent Antibody Test. Experimental Parasitology 35, 374380. A schizont antigen for the indirect fluorescent antibody test was prepared from an in &TO culture suspension of lymphoid cells infected with Thei...

BACKGROUND Bovine theileriosis results from infection with obligate intracellular protozoa of the genus Theileria. The phylogenetic relationships between two isolates of Theileria annulata, and 36 Theileria spp., as well as 6 outgroup including Babesia spp. and coccidian protozoa were analyzed using the 18S rRNA gene sequence. METHODS The target DNA segment was amplified by PCR. The PCR produ...

The present study was carried out to determine the prevalence of Theileria annulata in large ruminants from two districts, Peshawar and Kohat, in Khyber Pukhtoon Khwa (Pakistan). Blood samples were collected from 95 cattle. Data on the characteristics of animals and herds were collected through questionnaires. No significant risk factors were found associated with the spread of tropical theiler...

Theileria annulata, which is part of the Theileria sergenti/Theileria buffeli/Theileria orientalis group, preferentially infects cattle and results in high mortality and morbidity in the Mediterranean, Middle East, and Central Asia. The polypeptide Tams1 is an immunodominant major merozoite piroplasm surface antigen of T. annulata that could be used as a marker for epidemiological studies and p...

Theileria annulata and T. parva are closely related protozoan parasites that cause lymphoproliferative diseases of cattle. We sequenced the genome of T. annulata and compared it with that of T. parva to understand the mechanisms underlying transformation and tropism. Despite high conservation of gene sequences and synteny, the analysis reveals unequally expanded gene families and species-specif...

BACKGROUND The present study was carried out to investigate the accurate status of ovine Theileria infection in sheep from Ahvaz and surrounding region, a tropical area southwest Iran. METHODS A PCR-RFLP method based on 18S ribosomal RNA gene was designed which could detect and differentiate Theileria and Babesia spp. and also differentiate main Theileria species in sheep at the same time. 11...